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Sample GSM66322 Query DataSets for GSM66322
Status Public on Jan 01, 2006
Title Saccharomyces cerevisiae Sgu52 NS24 EM93 NS24 Array_B
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae Sgu52 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae Sgu52 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy5
Label protocol See Townsend, Cavalieri and Hartl 2003
 
Channel 2
Source name Saccharomyces cerevisiae EM93 in NS24
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae EM93 in NS24
Extracted molecule polyA RNA
Extraction protocol See Townsend, Cavalieri and Hartl 2003
Label Cy3
Label protocol See Townsend, Cavalieri and Hartl 2003
 
 
Scan protocol
Description Some hybridizations were performed in duplicates on the same microarrays. Two complete sets of PCR products were printed on the same glass slide and under a single cover slip. These duplicates are labeled Array_A and Array_B. Since these are not complete technical replicates, they were averaged in Landry et al. (Gene, accepted) following the normalization and prior to statistical analysis. Replicate indicates a complete independent hybridization.
Data processing Spots of bad quality were given negative flags. Nonnormalized ratios are provided.
 
Submission date Jul 27, 2005
Last update date Jul 28, 2005
Contact name Duccio Cavalieri
E-mail(s) [email protected]
Phone 39.055.4271327
Fax 39.055.4271280
Organization name University of Florence
Department Department of Pharmacology
Street address Viale Pieraccini 6
City Florence
ZIP/Postal code 50139
Country Italy
 
Platform ID GPL2682
Series (1)
GSE3021 Six diploid strains grown in four conditions

Data table header descriptions
ID_REF Platform ID
VALUE log ratio of median F635 divided by F532
F635 Median
B635 Median
F532 Median
B532 Median
Flags zero signififies pass, less than zero is a bad spot

Data table
ID_REF VALUE F635 Median B635 Median F532 Median B532 Median Flags
1 -0.71 105 55 172 114 0
2 -1.36 115 36 295 76 0
3 -0.51 1675 45 2379 95 0
4 -1.34 302 41 764 103 0
5 -3.30 2687 48 26423 106 0
6 -1.43 206 40 555 107 0
7 -0.88 1071 39 1976 81 0
8 -0.79 206 36 356 75 0
9 -0.24 403 49 477 96 0
10 -0.96 151 39 294 86 0
11 -3.19 232 45 2113 98 0
12 -1.41 533 39 1419 92 0
13 -1.26 522 45 1249 96 0
14 -1.62 246 42 755 110 0
15 -0.98 1032 48 2030 112 0
16 -1.28 335 39 816 92 0
17 -0.97 708 49 1384 99 0
18 -1.29 441 37 1080 78 0
19 -0.88 958 43 1758 89 0
20 -1.34 524 41 1327 110 0

Total number of rows: 6218

Table truncated, full table size 169 Kbytes.




Supplementary data files not provided

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