|
| Status |
Public on Oct 06, 2022 |
| Title |
Oral-aboral 6 [z06] |
| Sample type |
SRA |
| |
|
| Source name |
sectioned from whole arm
|
| Organism |
Patiria miniata |
| Characteristics |
tissue: sectioned from whole arm
isolate: specimen 1
|
| Treatment protocol |
arm embedded in HistoPrep medium and cryosectioned using Leica CM3050-S microtome
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA extracted using qiagen RNAeasy and modified Trizol protocol
Iso-Seq Express Template Preparation protocol / SMRTbell Express Template Prep Kit 2.0
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Sequel II |
| |
|
| Data processing |
demultiplex with Lima
isoseq 3.0
mapped full length transcripts with minmap2
counted transcript hits to reference transcriptome from SAM file
Assembly: TBD
Supplementary files format and content: counts.txt
|
| |
|
| Submission date |
Oct 03, 2022 |
| Last update date |
Oct 06, 2022 |
| Contact name |
Adam Knight |
| E-mail(s) |
[email protected]
|
| Phone |
5103979735
|
| Organization name |
pacbio
|
| Street address |
1305 Obrien Dr
|
| City |
Menlo Park |
| State/province |
CA |
| ZIP/Postal code |
94025 |
| Country |
USA |
| |
|
| Platform ID |
GPL32720 |
| Series (1) |
| GSE214723 |
Three dimensional sectioning and full length gene expression profiling of Patiria miniata |
|
| Relations |
| BioSample |
SAMN30501235 |
| SRA |
SRX17786543 |
