|
| Status |
Public on Sep 30, 2022 |
| Title |
Regulatory small RNA CRISPR flamΔZAM |
| Sample type |
SRA |
| |
|
| Source name |
ovaries
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: ovaries
genotype: CRISPR flam-delta-ZAM
time: 3-6 day old female
|
| Growth protocol |
Flies were kept at standard conditon at 25°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Argonaute-bound sRNAs were isolated with the TRApR kit according to Grentzinger et al. (2020)
gel-free protocol without size selection - Library preparation using QIAseq-miRNA kit
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Enriched small RNA
|
| Data processing |
Adapters were trimmed and size selection was performed to retain only 18-29 nucleotide reads.
|
| |
|
| Submission date |
Sep 14, 2022 |
| Last update date |
Sep 30, 2022 |
| Contact name |
Emilie BRASSET |
| E-mail(s) |
[email protected]
|
| Organization name |
CNRS 6293 INSERM U1103 Université Clermont Auvergne
|
| Lab |
iGReD
|
| Street address |
28 place Henri dunant
|
| City |
CLERMONT-FERRAND |
| ZIP/Postal code |
63000 |
| Country |
France |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE213368 |
Germline piRNAs counteract endogenous retrovirus invasion from somatic cells- small RNA |
|
| Relations |
| BioSample |
SAMN30855344 |
| SRA |
SRX17578054 |
