|
| Status |
Public on Jan 21, 2011 |
| Title |
27hpf ATG 1 vs 27hpf StCo 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
hdac1 ATG morphant
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Whole embryo
age: 27 hours post fertilisation
morphant: hdac1 ATG morphant
|
| Growth protocol |
Embryos injected with morpholino where cultured in E3 at 28.5°C for 27 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol, treated with Dnase and purified using QIAgen RNA columns all following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Ambion Amino Allyl MessageAmp II aRNA Amplification Kit and Perkin Elmer Cy-dye Coupling
|
| |
|
| Channel 2 |
| Source name |
Standard control morphant
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Whole embryo
age: 27 hours post fertilisation
morphant: Standard control morphant
|
| Growth protocol |
Embryos injected with morpholino where cultured in E3 at 28.5°C for 27 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol, treated with Dnase and purified using QIAgen RNA columns all following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Ambion Amino Allyl MessageAmp II aRNA Amplification Kit and Perkin Elmer Cy-dye Coupling
|
| |
|
| |
| Hybridization protocol |
Two-Color Microarray-Based Gene Expression Analysis Protocol (G4140-90050, Agilent Technologies, Palo Alto, USA)
|
| Scan protocol |
Two-Color Microarray-Based Gene Expression Analysis Protocol (G4140-90050, Agilent Technologies, Palo Alto, USA)
|
| Description |
Biological replicate 1 of 2 Hdac1 ATG injected embryo
|
| Data processing |
Agilent Feature Extraction Software and Rosetta Resolver was used for background subtraction and normalization.
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Jan 21, 2011 |
| Contact name |
Michael Harrison |
| E-mail(s) |
[email protected]
|
| Phone |
+441142222381
|
| Organization name |
University of Sheffield
|
| Department |
MRC Centre for Developmental and Biomedical Genetics
|
| Lab |
Cunliffe
|
| Street address |
Firth Court, Western Bank
|
| City |
Sheffield |
| ZIP/Postal code |
S10 2TN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL7735 |
| Series (3) |
| GSE26708 |
Zebrafish embryos: hdac1 Morphants vs Standard control morphants |
| GSE26709 |
Zebrafish embryos: hdac1 Morphants vs Standard control morphants at 12, 18 and 27 hpf |
| GSE26710 |
Zebrafish hdac1 mutant and morphant embryos |
|
