|
| Status |
Public on Sep 10, 2022 |
| Title |
EAU |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
age: 10 week
disease status: EAU mice
cell type: CD4+ T cells from spleen and draining lymph nodes
|
| Treatment protocol |
For induction of EAU, female C57BL/6 mice were injected intraperitoneally (i.p.) with 800 ng of pertussis toxin and immunized subcutaneously (s.c.) at over six spots on the tail base and flank with uveitogenic peptide IRBP1–20 emulsified in CFA containing mycobacterium tuberculosis strain H37RA (0.8 mg per mouse).
|
| Growth protocol |
CD4+ T cells were isolated and purified from the spleen and draining lymph nodes of naive and EAU mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the CD4+ T cells of Naive and EAU mice with TRIzol reagent.
|
| Label |
Cy3
|
| Label protocol |
miRNA in total RNA was labeled by miRNA Complete Labeling and Hyb Kit following the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Each slide was hybridized with Cy3-labeled RNA using miRNA Complete Labeling and Hyb Kit (Agilent) in hybridization Oven (Agilent) at 55°C, 20 rpm for 20 hours according to the manufacturer’s instructions. After hybridization, slides were washed by Gene Expression Wash Buffer Kit (Agilent).
|
| Scan protocol |
Slides were scanned by Agilent Microarray Scanner (Cat # G2565CA, Agilent technologies, Santa Clara, CA, US).
|
| Data processing |
The scanned images were analyzed with Feature Extraction software 10.7 (Agilent).
|
| |
|
| Submission date |
Sep 09, 2022 |
| Last update date |
Sep 10, 2022 |
| Contact name |
hong nian |
| E-mail(s) |
[email protected]
|
| Organization name |
Tianjin Medical University Eye Hospital
|
| Street address |
251 Fukang Road, Nankai District
|
| City |
Tianjin, China |
| ZIP/Postal code |
300384 |
| Country |
China |
| |
|
| Platform ID |
GPL17912 |
| Series (1) |
| GSE213034 |
Murine CD4+ T cells: naive vs. experimental autoimmune uveitis |
|
