|
| Status |
Public on Jan 07, 2011 |
| Title |
Keratinocytes, untreated 48h, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Epidermal keratinocytes, untreated control, 48h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary epidermal keratinocytes
treatment: none
treatment duration: 48h
|
| Treatment protocol |
~70% confluent keratinocytes were treated with 0.1μM DEX (Sigma).
|
| Growth protocol |
Standard growth protocol (e.g. as in Banno et al., J Biol Chem 279(31): 32633-32642 (PMID 15145954)).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard extraction protocol (e.g. as in Banno et al., J Biol Chem 279(31): 32633-32642 (PMID 15145954)).
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
Ctrl 48hrs-exp I
|
| Data processing |
MAS5 GCOS, RMAExpress summarized using PLM and log2 transformed.
|
| |
|
| Submission date |
Jan 06, 2011 |
| Last update date |
Jan 07, 2011 |
| Contact name |
Miroslav Blumenberg |
| E-mail(s) |
[email protected]
|
| Phone |
212 263-5924
|
| Organization name |
NYU School of Medicine
|
| Department |
Dermatology
|
| Lab |
Blumenberg
|
| Street address |
455 First Ave #874
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE26487 |
Effects of Glucocorticoids in Epidermal Keratinocytes |
|
