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Sample GSM64924 Query DataSets for GSM64924
Status Public on Jul 22, 2005
Title R1 ES 0h 2, chip MOE430A
Sample type RNA
 
Source name R1, Fetal cells
Organism Mus musculus
Characteristics Strain: 129/Sv x 129/Sv-CP
Gender: Male
Biomaterial provider Contact details for sample vendor: R1 sample obtained from Andras Nagy's lab
Growth protocol Growth medium: ES Cell Medium - DMEM (high glucose)
Media supplements: Hyclone FCS (heat inactivated), non-essential amino acids, Glutamax, Penicillin/Streptomycin, sodium pyruvate, beta-mercaptoethanol, LIF
Culture conditions: 37 C, 5% CO2
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Cell purification method: Culturing
Contaminating cell type: None
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki ([email protected]; 501 Smyth Road) for analysis.
Stembase Experiment ID: E165
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=165
SCGP Sample ID: S206
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=165#SAMPLE_165
Short description: 0 hours of an 11-point time course study of R1 embryonic stem cells and their cognate differentiated embryoid bodies. All samples investigated in triplicate. The cells are differentiating embryoid bodies from murine embryonic stem cells.
Estimated purity: 100%
RNA concentration: 2.0 ug/uL
Num cells for RNA prep: Unable to break up embryoid bodies to do a cell count.
Sample volume: 15.0 uL
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Jul 21, 2005
Last update date Apr 01, 2011
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL339
Series (1)
GSE2972 R1 Embryonic Stem Cell and Embryoid Body Time Course
Relations
Reanalyzed by GSE26498

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 2852.23 P 0.00227496
AFFX-BioB-M_at 6644.66 P 0.000340305
AFFX-BioB-3_at 2834.88 P 0.000340305
AFFX-BioC-5_at 6403.19 P 0.000146581
AFFX-BioC-3_at 5025.52 P 4.42873e-05
AFFX-BioDn-5_at 4656.18 P 4.42873e-05
AFFX-BioDn-3_at 38976.1 P 0.000169227
AFFX-CreX-5_at 65739.8 P 4.42873e-05
AFFX-CreX-3_at 104888 P 4.42873e-05
AFFX-DapX-5_at 33623.4 P 4.42873e-05
AFFX-DapX-M_at 59906.3 P 5.16732e-05
AFFX-DapX-3_at 79675.4 P 4.42873e-05
AFFX-LysX-5_at 7714.92 P 4.42873e-05
AFFX-LysX-M_at 11835.3 P 4.42873e-05
AFFX-LysX-3_at 21738.8 P 5.16732e-05
AFFX-PheX-5_at 3108.05 P 0.000146581
AFFX-PheX-M_at 3398.33 P 0.000389797
AFFX-PheX-3_at 4196.57 P 6.02111e-05
AFFX-ThrX-5_at 1340.78 P 0.00179591
AFFX-ThrX-M_at 1516.69 P 0.00359458

Total number of rows: 22690

Table truncated, full table size 691 Kbytes.




Supplementary file Size Download File type/resource
GSM64924.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM64924.EXP.gz 498 b (ftp)(http) EXP

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