|
| Status |
Public on Jan 20, 2012 |
| Title |
KOSTIM2 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from murine bone-marrow derived macrophages
|
| Organism |
Mus musculus |
| Characteristics |
treatment: LPS
cell type: murine bone-marrow derived macrophages
genotype: NFAT5 KO
genetic background: 129sv
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Kit QIAGEN
|
| Label |
Biotin
|
| Label protocol |
100-300 ng of total RNA were amplified and labeled using the WT Sense Target labeling system and control reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
Sample was hybridized to Mouse Gene 1.0 ST Array (Affymetrix) using a GeneChip Hybridization Oven 640
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 7G
|
| Description |
Gene expression data from murine bone-marrow derived macrophages
|
| Data processing |
The data were analyzed using the RMA algorithm and then SAM was applied to calculate significant differential expression between samples
|
| |
|
| Submission date |
Dec 29, 2010 |
| Last update date |
Jan 20, 2012 |
| Contact name |
Cristina Lopez-Rodriguez |
| E-mail(s) |
[email protected]
|
| Phone |
+34 93 316 0810
|
| Organization name |
Universitat Pompeu Fabra
|
| Department |
Medicine and Life Sciences
|
| Lab |
Immunology
|
| Street address |
Carrer Dr. Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26343 |
Gene expression from bone-marrow derived macrophages. |
|
