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| Status |
Public on Aug 13, 2022 |
| Title |
A_0_1 |
| Sample type |
SRA |
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| Source name |
Fungal hyphae cells
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| Organism |
Orbilia oligospora ATCC 24927 |
| Characteristics |
strain: ATCC 24927
synonymn: Arthrobotrys oligospora
cell type: Fungal hyphae cells
genotype: wild type
treatment: no treatment
time: --
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| Growth protocol |
The wild-type and mutant strains were cultured on potato dextrose agar (PDA) medium at 28°C.
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissue samples, the concentration and purity of the extracted RNA were detected by Nanodrop2000, RNA integrity was detected by agarose gel electrophoresis, and RIN value was determined by Agilent2100. A single library construction requires that the total amount of RNA is ≥1ug, the concentration is ≥35ng/μL, OD260/280 is 1.8 - 2.2, and OD260/230≥1.0.
The libraries were prepared for sequencing using standard Illumina protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
The data from high-throughput sequencing were analyzed using the Majorbio tools, a free online platform for data analysis (www.majorbio.com).
Assembly: HiSat2 and StringTie
Supplementary files format and content: xls file includes tpm value for each sample
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| Submission date |
Aug 10, 2022 |
| Last update date |
Aug 13, 2022 |
| Contact name |
Meichen Zhu |
| E-mail(s) |
[email protected]
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| Phone |
18883340353
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| Organization name |
yunnandaxue
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| Department |
School of Life Sciences
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| Lab |
Yang lab
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| Street address |
kunmingshi
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| City |
kunming |
| State/province |
Yunnan |
| ZIP/Postal code |
650000 |
| Country |
China |
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| Platform ID |
GPL32558 |
| Series (1) |
| GSE210886 |
The cAMP-PKA signaling pathway regulates hyphal growth, conidiation, trap morphogenesis, stress tolerance and autophagy in Arthrobotrys oligospora |
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| Relations |
| BioSample |
SAMN30227595 |
| SRA |
SRX17018685 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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