|
| Status |
Public on Dec 18, 2010 |
| Title |
Patient 999, 24 month of BETAFERON |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 50
cell type: PBMC
individual: Patient 999
treatment: BETAFERON
time: 24 month of BETAFERON
disease state: multiple sclerosis
nab status: NAB+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from fresh peripheral blood mononuclear cells using an RNeasy kit (Quiagen, Santa Clarita, USA)
|
| Label |
biotin
|
| Label protocol |
Samples were chemically fragmented and biotinylated using the IVT Labelling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Chips were processed on an Affymetrix GeneChip Fluidics Station 450. Background adjustment by Robust Multi-Array (RMA) algorithm was applied to probes intensities. Raw probes gene level expression were summarized from the corrected intensities and then quantile normalized.
|
| |
|
| Submission date |
Dec 16, 2010 |
| Last update date |
Jun 13, 2012 |
| Contact name |
Ramil Nurtdinov |
| E-mail(s) |
[email protected]
|
| Organization name |
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
|
| Department |
Bioinformatics and Genomics
|
| Lab |
Computational Biology of RNA Processing
|
| Street address |
Dr. Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE26104 |
Search for specific biomarkers of IFN-beta bioactivity in patients with MS |
|
