|
| Status |
Public on Nov 30, 2011 |
| Title |
231BrM-2a CD24-/CD44+/ESA+ population, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
CD24-/CD44+/ESA+ population in breast cancer cell line 231BrM-2a
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 231BrM-2a
|
| Treatment protocol |
Stem cell population (CD24-/CD44+/ESA+) was isolated by MACS system (Miltenyi) using CD24, CD44 and ESA antibodies.
|
| Growth protocol |
Cells were grown in RPMI-1640 media with 10% FBS and antibiotics in 4% CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using Qiagen RNeasy kit followed by DNase treatment and repulified by using Qiagen RNA cleanup kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix WT protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, approx 2 ug ss cDNA were hybridized for 16 hr at 45C on Affymetrix Human Gene 1.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from cancer stem cell population in breast cancer cell line
|
| Data processing |
The data were analyzed with RMA using Affymetrix Expression Console Software.
|
| |
|
| Submission date |
Dec 09, 2010 |
| Last update date |
Nov 30, 2011 |
| Contact name |
Hiroshi Okuda |
| E-mail(s) |
[email protected]
|
| Phone |
217-545-3969
|
| Fax |
217-545-3227
|
| Organization name |
Southern Illinois University School of Medicine
|
| Department |
Med. Microbiol. Immunol. & Cell Biol.
|
| Street address |
825 N. Rutledge
|
| City |
Springfield |
| State/province |
IL |
| ZIP/Postal code |
62702 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE25976 |
Expression profiles of CD24-/CD44+/ESA+ population in MDA-MB-231 and its highly metastatic variants. |
|
