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Sample GSM63696 Query DataSets for GSM63696
Status Public on Sep 04, 2005
Title phyAphyBcry1cry2D 8K
Sample type RNA
 
Source name phyAphyBcry1cry2 quadruple phortoreceptor mutant dark grown seedlings. Ecotype Lansberg erecta
Organism Arabidopsis thaliana
Characteristics The alleles used for the generation of the quadruple mutant were phyA-201 ,phyB-5, cry1 (hy4) and cry2 (fha)from Ler background. phyAphyBcry1cry2 seeds were sown on 0.8% (w/v) agar in clear plastic boxes and incubated at 6 degrees for 5 d. Chilled seeds were exposed to 8 h of R at 22 degrees to induce homogeneous seed germination. After 3 d in full D at 22 degrees seedlings were harvested in liquid nitrogen and total RNA was extracted by using the RNEASY Plant mini kit (Qiagen).
Treatment protocol phyAphyBcry1cry2 seeds were incubated at 6 degrees for 5 d. Chilled seeds were exposed to 8 h of R at 22 degrees to induce homogeneous seed germination. After 3 d in full D at 22 degrees seedlings were harvested
Growth protocol seeds were sown on 0.8% (w/v) agar
Extracted molecule total RNA
Extraction protocol Rneasy plant mini kit (qiagen)
Label Affymetrix instructions
Label protocol Affymetrix instructions
 
Hybridization protocol Affymetrix instruction. Hybridization to 8 K Affymetrix Arabidopsis Gene Chips
Scan protocol Affymetrix instructions
Description For more information see Mazzella et al, 2005. Plant Cell Vol 17 (september)
Data processing the scaling tab of the Affymetrix microarray suite in the mode “all probe sets” was used to standardize the trimmed mean signal of each array to the “target signal” according to manufacturer instructions.
 
Submission date Jul 11, 2005
Last update date Oct 28, 2005
Contact name Maria Agustina Mazzella
E-mail(s) [email protected]
Phone 54-11-47832871
Organization name INGEBI
Street address Vuelta de Obligado 2490
City Capital Federal
State/province Buenos AIres
ZIP/Postal code 1428
Country Argentina
 
Platform ID GPL2598
Series (1)
GSE2919 Transcriptome profiling of dark grown photoreceptor mutants of Arabidopsis.

Data table header descriptions
ID_REF
VALUE Final calculated measurement for each probe set identifier that has been made comparable across all samples and rows

Data table
ID_REF VALUE
11986_AT (X96598.1_AT) 148.1
11987_AT (AL021961.134_AT) 179.2
11988_AT (AC002328.16_AT) 49.8
11989_AT (AC002328.26_AT) 18.1
11990_AT (AC002387.210_AT) 16.9
11991_G_AT (AC002387.210_G_AT) 44
11992_AT (AC002396.18_AT) 62.1
11993_AT (AC003027.19_AT) -0.9
11994_AT (AC004218.86_AT) 15.9
11995_AT (AC004680.130_AT) 11
11996_AT (AC005770.145_AT) 66.7
11997_AT (AC005967.4_AT) 52.9
11998_AT (AC006341.63_AT) 31.1
11999_AT (AC007195.115_AT) 1.3
12000_AT (AC007202.40_AT) 37.6
12001_AT (AC007591.48_AT) 21.6
12002_AT (AF069442.47_AT) 92.6
12003_AT (AJ012459.2_AT) -4.9
12004_AT (AL022023.132_AT) 509.6
12005_AT (AL035678.251_AT) 3

Total number of rows: 8775

Table truncated, full table size 269 Kbytes.




Supplementary data files not provided
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