|
| Status |
Public on Dec 07, 2010 |
| Title |
Brain CD8 T cells that are IL-10+ rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Directly ex vivo brain CD8 T cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain
strain: C57/BL6 Vert-X
genotype: IL-10eGFP
age: 6-8 weeks
cell type: CD8 T cells that are IL-10+
|
| Treatment protocol |
These CD8 T lymphocytes are naturally IL-10+ and IL-10-.
|
| Growth protocol |
C57Bl/6 Vert-X (IL-10-eGFP) mice were infected intracranially with 500 PFU J2.2-V-1 virus and seven days later their brain lymphocytes were isolated and negatively selected for CD8 T cells. The CD8 brain lymphocytes were then sorted for IL-10+ and IL-10- CD8 T lymphocytes using GFP as a marker.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was achieved by purification on RNeasy columns.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared using NuGEN WT-Ovation PicoRNA amplification system, starting from 800 pg to 50 ng of total RNA.
|
| |
|
| Hybridization protocol |
2.5 ug of labeld cDNA were hybridized on mouse GENE 1.0 ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner 3000 complete with a 7G upgrade.
|
| Description |
Gene expression data from IL-10+CD8 T cells in a J2.2-V-1-infected mouse
|
| Data processing |
The data were analyzed with Partek GS v 6.4 using default RMA as normalization method.
|
| |
|
| Submission date |
Dec 03, 2010 |
| Last update date |
Dec 06, 2010 |
| Contact name |
Stanley Perlman |
| E-mail(s) |
[email protected]
|
| Phone |
3193358549
|
| Organization name |
University of Iowa
|
| Department |
Department of Microbiology
|
| Street address |
51 Newton Road
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE25846 |
Expression data from IL-10+ and IL-10- CD8 T cells |
|
