|
| Status |
Public on Sep 01, 2011 |
| Title |
3036 |
| Sample type |
RNA |
| |
|
| Source name |
KrasG12D Pten L/+ PDEC
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/C57B6 mixed backgroud
cell type: primary KrasG12D Pten L/+ pancreatic ductal epithelial cells, P4
genotype/variation: Pdx1-Cre;LSL-KrasG12D L/+;Pten L/+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA followed by Rneasy column cleanup was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The software used was Expression Console from Affimatrix and normalization algorithm used was MAS5.
|
| |
|
| Submission date |
Dec 02, 2010 |
| Last update date |
Sep 01, 2011 |
| Contact name |
Haoqiang Ying |
| E-mail(s) |
[email protected]
|
| Phone |
617-582-7978
|
| Fax |
617-632-6069
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Lab |
Ronald DePinho
|
| Street address |
44 Binney St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE25828 |
Pten deficiency cooperates with KrasG12D to activate NFkB pathway promoting the development of malignant pancreatic ductal adenocarcinoma |
|
