|
Status |
Public on Dec 03, 2010 |
Title |
G2019S-MAN |
Sample type |
RNA |
|
|
Source name |
fibroblasts
|
Organism |
Homo sapiens |
Characteristics |
cell type: patient derived fibroblast cells disease status: parkinson's disease lrrk2 mutation: G2019S
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
PD
|
Data processing |
The data were normalised using quantile normalisation with Beadstudio v3.2
|
|
|
Submission date |
Nov 23, 2010 |
Last update date |
Nov 23, 2010 |
Contact name |
Marcel van der Brug |
Organization name |
NIH
|
Department |
NIA
|
Lab |
Neurogenetics
|
Street address |
9000 Rockville Pike
|
City |
Bethesda |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL6947 |
Series (1) |
GSE25580 |
Examination of impact of pathogenic mutations in LRRK2 upon gene expression in patient fibroblasts and HEK cells |
|