|
| Status |
Public on Dec 03, 2010 |
| Title |
RC1 12h 89 |
| Sample type |
RNA |
| |
|
| Source name |
Stable iLRRK2 HEK Cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK
transfectant: LRRK2 with the R1441C mutation
treatment time: 12 hr
|
| Treatment protocol |
The Flp-In-Trex vector was used to generate stable cell lines expressing the lrrk2 gene. Doxycyclin (1ug/ml final conc) was used to induce expression.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
RC 12Hr
|
| Data processing |
The data were normalised using quantile normalisation with Beadstudio v3.2
|
| |
|
| Submission date |
Nov 23, 2010 |
| Last update date |
Nov 23, 2010 |
| Contact name |
Marcel van der Brug |
| Organization name |
NIH
|
| Department |
NIA
|
| Lab |
Neurogenetics
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE25580 |
Examination of impact of pathogenic mutations in LRRK2 upon gene expression in patient fibroblasts and HEK cells |
|
