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Sample GSM628640 Query DataSets for GSM628640
Status Public on Dec 03, 2010
Title RC1 0h 81
Sample type RNA
 
Source name Stable iLRRK2 HEK Cells
Organism Homo sapiens
Characteristics cell line: HEK
transfectant: LRRK2 with the R1441C mutation
treatment time: 0 hr
Treatment protocol The Flp-In-Trex vector was used to generate stable cell lines expressing the lrrk2 gene. Doxycyclin (1ug/ml final conc) was used to induce expression.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description RC 00Hr
Data processing The data were normalised using quantile normalisation with Beadstudio v3.2
 
Submission date Nov 23, 2010
Last update date Nov 23, 2010
Contact name Marcel van der Brug
Organization name NIH
Department NIA
Lab Neurogenetics
Street address 9000 Rockville Pike
City Bethesda
ZIP/Postal code 20892
Country USA
 
Platform ID GPL6104
Series (1)
GSE25580 Examination of impact of pathogenic mutations in LRRK2 upon gene expression in patient fibroblasts and HEK cells

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 1079.134 44 48.07867 0
ILMN_1660305 534.4806 36 26.36006 0
ILMN_1792173 1017.06 38 45.59132 0
ILMN_1762337 69.37189 40 3.42344 0.7398844
ILMN_1736007 74.67053 46 4.906431 0.5621387
ILMN_1787689 96.97857 42 5.37094 0.05924856
ILMN_1731507 67.64128 24 4.628746 0.8106936
ILMN_1814316 76.8172 31 4.709335 0.4653179
ILMN_1745607 71.069 38 5.239966 0.6907514
ILMN_1757454 86.75256 45 4.599368 0.1950867
ILMN_1735045 88.62431 52 4.685975 0.1560694
ILMN_1680754 119.7592 38 6.795091 0
ILMN_1659452 63.23919 36 2.892688 0.9263006
ILMN_1767388 138.752 40 7.443491 0
ILMN_1673870 81.98582 32 5.927248 0.3135838
ILMN_1675204 83.17516 39 5.682751 0.2817919
ILMN_1755321 316.6793 45 14.95924 0
ILMN_1698554 438.1401 33 25.85382 0
ILMN_1805938 81.90097 26 6.608175 0.3135838
ILMN_1676336 169.2239 35 10.29153 0

Total number of rows: 22184

Table truncated, full table size 854 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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