The tissues were maintained at -70°C prior to extraction
Extracted molecule
total RNA
Extraction protocol
Total RNA extraction was performed by using the miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol.
Label
Cy3
Label protocol
Agilent’s miRNA Complete Labeling and Hybridization Kit V2 (Protocol Version 2.1, Agilent Technologies, Palo Alto, CA, USA) was utilized for processing the samples.
Hybridization protocol
Agilent’s miRNA Complete Labeling and Hybridization Kit V2 (Protocol Version 2.1, Agilent Technologies, Palo Alto, CA, USA) was utilized for processing the samples.
Scan protocol
High-resolution Microarray Scanner (G2565CA, Agilent Technologies, Palo Alto, CA, USA) was used to scan the microarrays and the images were processed with Agilent Feature Extraction Software v. 9.5.
Description
asbestos-fiber_count 2.8
Data processing
Images were processed with Agilent Feature Extraction Software v. 9.5. The data was analyzed using Chipster version 1.4.2 (http://chipster.csc.fi/).
