|
| Status |
Public on Jun 30, 2005 |
| Title |
25% whole mouse 75% mouse colon |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6 whole mouse/mouse colons
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6 Tissue: whole mouse/mouse colon Sample: pooled
|
| Biomaterial provider |
n/a
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
from 10ng of total RNA
|
| Label |
according to Affymetrix' recommendations
|
| Label protocol |
T7 double amplification protocol (Schwab et al., 2003)
|
| |
|
| Hybridization protocol |
according to Affymetrix' recommendations
|
| Scan protocol |
according to Affymetrix' recommendations
|
| Description |
Two reference RNA samples were prepared, one from 8 week old adult C57BL/6 mouse colons and one from one day old C57BL/6 whole pups by homogenizing tissue in Trizol reagent (Invitrogen) with a Polytron tissue homogenizer (Brinkman, Westbury, NY). Both samples were prepared as a pooled single sample from multiple animals. RNA was purified following the manufacturer’s recommendations and integrity was analyzed using an Agilent BioAnalyzer 2100 according to manufacturer instructions. A dilution series of five sample RNAs was prepared from the two reference RNAs by mixing the one day old whole mouse RNA and the 8-week old colon RNA.
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jun 28, 2005 |
| Last update date |
Oct 28, 2005 |
| Contact name |
Johannes Michael Freudenberg |
| E-mail(s) |
[email protected]
|
| Phone |
(+1)513-636-1424
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Biomedical Informatics
|
| Lab |
Bruce Aronow
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE2850 |
MOE430 whole mouse - mouse colon dilution series - RMA preprocessed |
|
