|
| Status |
Public on Nov 23, 2010 |
| Title |
CD8 KLF13 WT 1 |
| Sample type |
RNA |
| |
|
| Source name |
CD8 KLF13 WT
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
cell type: CD8 T
genotype/variation: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with RNAeasy kit from Qiagen RNeasy. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
|
| Label |
biotin
|
| Label protocol |
200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Four replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse Gene ST 1.0 GeneChip
|
| Scan protocol |
scanned on Affymetrix GeneChip scanner 3000. Data were collected using Affymetrix GCOS software.
|
| Data processing |
Statistical and clustering analysis was performed with Partek Genomics Suite software using RMA normalization algorithm. Differentially expressed genes were identified with ANOVA analysis. Genes that are up- or down-regulated more than 2 fold and with a p<0.001 were considered significant. Significant genes were analyzed for enrichment for pathways using DAVID bioinformatics database (http://david.abcc.ncifcrf.gov/) and Ingenuity Pathway Analysis software.
|
| |
|
| Submission date |
Nov 19, 2010 |
| Last update date |
Nov 22, 2010 |
| Contact name |
Dazhi Lai |
| Organization name |
NIH
|
| Department |
NHLBI
|
| Lab |
LMI
|
| Street address |
10 Center Dr, 7B20A
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE25502 |
KLF13 regulate memory-like CD8 T cells |
|
