|
| Status |
Public on Aug 13, 2012 |
| Title |
WAT_Obese [1529] |
| Sample type |
RNA |
| |
|
| Source name |
Abdominal subcutaneous adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: White adipose tissue
genotype: female
age: 45
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Nucleospin RNA11 kit (Macherey-Nagel, Duren, Germany). RNA samples were treated with RNase-free DNase (Macherey-Nagel).
|
| Label |
biotin
|
| Label protocol |
Total RNA were amplified and biotin labeled using the Whole Transcript (WT) Sense Target Labeling Protocol
|
| |
|
| Hybridization protocol |
From non-degraded high-quality total RNA we prepared and hybridized biotinylated complementary RNA to Gene 1.0 ST Arrays, and then washed, stained and scanned slides using standardized protocols (Affymetrix Inc., Santa Clara, CA).
|
| Scan protocol |
We used standardized Affymetrix protocols (Affymetrix Inc., Santa Clara, CA)
|
| Data processing |
Subsequent data analyses were performed using the Affymetrix GeneChip Operating Software (GCOS) Version 1.4. To allow comparisons of transcript levels between samples, all samples were subjected to an all-probe set scaling to target signal 100. # Summarization: PLIER, Background correction: PM-GCBG, Normalization method: Global Median
|
| |
|
| Submission date |
Nov 16, 2010 |
| Last update date |
Aug 14, 2012 |
| Contact name |
Ingrid Dahlman |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska
|
| Street address |
Karolinska Huddinge
|
| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE25401 |
Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [gene expression] |
| GSE25402 |
Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity |
|
