|
| Status |
Public on Nov 22, 2022 |
| Title |
Ce-49 sh 3 |
| Sample type |
genomic |
| |
|
| Source name |
K562
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: ce49sh
disease: leukemia
|
| Treatment protocol |
1) PBS group received PBS (100 μL/injection); 2) AZA group received intra-tumoural injections of 5-Aza (Sigma) (1 mg/kg injection/mouse in 100 μL/injection); 3) Ce-49 sh group received intra-tumoural injections of Ce-49 sh loaded GenVoy LNPs (0.3 mg/kg injection/mouse in 100 μL/injection). Treatments were performed daily for 10 days.
|
| Growth protocol |
K562 cells grown in RPMI supplemented with 10% FBS and injected subcutaneously into the flank of NGS mice
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Qiagen DNeasy Kit according to standard instructions
|
| Label |
Standard Illumina
|
| Label protocol |
Standard Illumina Protocol by Diagenode
|
| |
|
| Hybridization protocol |
Standard Illumina Protocol by Diagenode
|
| Scan protocol |
Protocol by Diagenode
|
| Description |
genomic DNA from xenografted tumor
|
| Data processing |
The EPIC methylation array was analysed using the Bioconductor package RnBeads (v2.4.0) using the hg19 annotations.
|
| |
|
| Submission date |
Jun 07, 2022 |
| Last update date |
Nov 23, 2022 |
| Contact name |
Haiqing Li |
| E-mail(s) |
[email protected]
|
| Organization name |
City of Hope National Medical Center
|
| Department |
Shared Resources Department
|
| Lab |
Integrative Genomics Core Lab
|
| Street address |
1500 E Durate Rd
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL21145 |
| Series (2) |
| GSE205651 |
Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylation [MethylationEPIC] |
| GSE205655 |
Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylation |
|
