|
| Status |
Public on Mar 21, 2012 |
| Title |
Kasumi-1 leukemia cell line - 10nM DAC treated - day 21 [CH3'n profiling] |
| Sample type |
genomic |
| |
|
| Source name |
Kasumi-1 leukemia cell line, DAC treated, day 21
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Kasumi-1 leukemia cell line
agent: DAC
time point: day 21
|
| Treatment protocol |
10nM DAC for 72h, renewing the medium every 24h
|
| Growth protocol |
Obtained from ATCC and cultured according to instructions
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Phenol-chloroform extraction
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted (EZ DNA methylation kit) DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
kasumid21DAC
DAC treated cell line
|
| Data processing |
Raw methylated and unmethylated signals are used to recalculate beta values without background subtraction. Probes with a detection p-value equal to or greater than .05 are set to NA. Data handling and processing done in R.
|
| |
|
| Submission date |
Nov 05, 2010 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Leander Van Neste |
| Organization name |
Ghent University
|
| Department |
Molecular Biotechnology
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
Coupure Links 653
|
| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL8490 |
| Series (1) |
|
