|
| Status |
Public on Aug 31, 2024 |
| Title |
peripheral blood, colon polyps-r1 (RC347) |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
gender: M
age: 68
cancer dx: colon polyps
|
| Treatment protocol |
Blood plasma was removed after centrifugation and a hypotonic buffer was added at a 3:1 volume ratio to lyse the red blood cells. The mixture was centrifuged to yield a pellet for subsequent total RNA isolation.
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from the pelleted cells was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (GeneChip Expression Analysis Technical Manual, 701021 Rev. 4, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Human peripheral blood collected in EDTA blood collection tubes, total RNA isolated from remaining cells collected after red blood cell lysis.
Gene expression data from peripheral blood cells collected after red blood cell lysis.
|
| Data processing |
The data were analyzed with GeneChip Operating Software (GCOS) v1.1.1 using Affymetrix default analysis settings and global scaling (MAS 5.0) as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
May 14, 2022 |
| Last update date |
Aug 31, 2024 |
| Contact name |
Adam Dempsey |
| E-mail(s) |
[email protected]
|
| Phone |
804-655-6595
|
| Organization name |
StageZero Life Sciences
|
| Street address |
8751 Park Central Drive, Suite 200
|
| City |
Richmond |
| State/province |
VA |
| ZIP/Postal code |
23227 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE203024 |
Gene Expression Data from Human Peripheral Blood |
|
