|
| Status |
Public on Oct 30, 2010 |
| Title |
3 Day 18.5 |
| Sample type |
RNA |
| |
|
| Source name |
Day 18.5
|
| Organism |
Mus musculus |
| Characteristics |
tissue: uterus
developmental stage: 18.5 days post coitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mouse uterus was lysed in Trizol and mRNA was extracted using Qiagen RNA mini kit according to the manufacturers protocol.
|
| Label |
Cy3
|
| Label protocol |
Total mRNA labeling was performed by the UT Southwestern Microarray Core facility using Illumina labeling kit according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed by the UT Southwestern Microarray Core facility according to Illumina's protocol
|
| Scan protocol |
Scanning was performed by the UT Southwestern Microarray Core facility according to Illumina's protocol
|
| Description |
4444590083_F
|
| Data processing |
Data was initially processed using Illumina Bead studio software where it was quantile normalized and background subtracted. Downstream analysis was done using Bioconductor.
|
| |
|
| Submission date |
Oct 29, 2010 |
| Last update date |
Feb 11, 2014 |
| Contact name |
Carole R Mendelson |
| E-mail(s) |
[email protected]
|
| Phone |
214-648-2944
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Department |
Biochemistry
|
| Street address |
5323 Harry Hines Blvs
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-9038 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE25017 |
The miR-200 family and targets, ZEB1 and ZEB2, modulate uterine quiescence and contractility during pregnancy and labor |
|
