|
| Status |
Public on Aug 04, 2024 |
| Title |
HUVEC_control_48hours |
| Sample type |
RNA |
| |
|
| Source name |
HUVEC
|
| Organism |
Homo sapiens |
| Characteristics |
cell: HUVEC
stimulation: Captisol
|
| Treatment protocol |
HUVEC were precultured in EGM2 complete media for 24 hours, and then treated with 100µM Captisol or JPH203 (J-Pharma) for 48 hours.
|
| Growth protocol |
Human umbilical vein endothelial cells (HUVEC) were purchased from Ronza and cultured in EGM-2 complete medium (Ronza).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with TRIzol reagents (Invitrogen) according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
|
| |
|
| Hybridization protocol |
10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
|
| Scan protocol |
Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
|
| Description |
Gene expression data from HUVEC treated with 100µM Captisol for 48 hours
|
| Data processing |
Affymetrix GeneChip Operating Software (GCOS) Version 1.0. with target intensity=100.
|
| |
|
| Submission date |
May 11, 2022 |
| Last update date |
Aug 04, 2024 |
| Contact name |
Jun-ichi Suehiro |
| Organization name |
Kyorin University School of Medicine
|
| Department |
Department of Pharmacology and Toxicology
|
| Street address |
6-20-2, Shinkawa
|
| City |
Mitaka |
| State/province |
Tokyo |
| ZIP/Postal code |
181-8611 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE202790 |
Transcriptome analysis in JPH203-treated HUVEC |
|
