|
| Status |
Public on Dec 06, 2010 |
| Title |
UGS at E17 from DES exposed fetus |
| Sample type |
RNA |
| |
|
| Source name |
DES-exposed, Mouse fetus UGS region at E17
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
agent: DES
age: E17
tissue: urogenital sinus
|
| Treatment protocol |
The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
|
| Growth protocol |
Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
|
| Label |
biotin
|
| Label protocol |
During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
|
| |
|
| Hybridization protocol |
Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
|
| Description |
Gene expression data from DES exposed fetus UGS at E17
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Oct 26, 2010 |
| Last update date |
Dec 06, 2010 |
| Contact name |
KATSUHIDE IGARASHI |
| E-mail(s) |
[email protected]
|
| Phone |
+81-3-3786-1011
|
| Organization name |
Hoshi University School of Pharmacy and Pharmaceutical Science
|
| Department |
Life Science Tokyo Advanced Research center (L-StaR)
|
| Street address |
2-4-41 Ebara
|
| City |
Shinagawa-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
142-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE24928 |
Gene expression change induced by bisphenol A in mouse urogenital sinus |
|
