|
| Status |
Public on Aug 05, 2022 |
| Title |
SP P 20 39-2_(SNOWBALLs520824F) |
| Sample type |
RNA |
| |
|
| Source name |
porcine myoblasts
|
| Organism |
Sus scrofa |
| Characteristics |
cell type: myoblasts
pool: P 20
temperature: 39 degrees C
|
| Treatment protocol |
The isolation of satellite cells from the M. rhomboideus of ten female five- and twenty days old piglets and the establishment and validation of two muscle cell pools (pool 5 (P5) or pool 20 (P20))
For proliferation experiments cells from both pools stored in liquid nitrogen were defrosted and permanently cultured at 35°, 37° (control), 39° or 41°C over a period of 72 h in growth medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
biotin-labeled cRNAs were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42 C on Affymetrix Porcine Snowball Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
|
| Data processing |
Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500.
|
| |
|
| Submission date |
May 10, 2022 |
| Last update date |
Aug 05, 2022 |
| Contact name |
Siriluck Ponsuksili |
| E-mail(s) |
[email protected]
|
| Organization name |
FBN
|
| Street address |
Wilhelm-stahl-allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16569 |
| Series (1) |
| GSE202678 |
Effects of temperature on transcriptome and energy metabolism of porcine myoblasts |
|
