|
| Status |
Public on Oct 19, 2011 |
| Title |
Sample 20 |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: part of liver
gender: male
age: 25 weeks
strain: transgenic mouse strain 40.6
date of exposure: March 2-30
exposure: Exposed to 75 mg/kg Bap
time post exposure: 3 days
|
| Treatment protocol |
25-week old male Muta™Mouse (i.e., transgenic mouse strain 40.6) were dosed daily via oral gavage for 28-days with BaP dissolved in olive oil (25, 50 and 75 mg/kg body weight/day) purchased from Sigma-Aldrich (Oakville, ON, Canada). Each dose group contained 5 animals; 5 animals were also dosed with a vehicle (olive oil) control. Following a 3 day expression period after the last treatment, mice were euthanized with isofluorane followed by cervical dislocation. Liver was then excised, flash-frozen in liquid nitrogen, and stored at -80ºC until use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA containing the small fraction was isolated from each sample using the miRVana miRNA Isolation Kit (Ambion), Streetsville, ON, Canada). Quality was confirmed using the Agilent 2100 Bioanalyzer and RNA Nano 6000 chips.
|
| Label |
Cyanine 3-pCp
|
| Label protocol |
Total RNA samples were labelled using the Agilent miRNA Complete Labelling and Hybridisation Kit (Agilent Tech, Mississauga, ON, Canada).
|
| |
|
| Hybridization protocol |
Hybridisations were performed in Agilent SureHyb chambers for 20 hours at 55C and washed according to manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned at a resolution of 5 mm using an Agilent G2505B scanner.
|
| Data processing |
Data were extracted from scanned images using Agilent Feature Extraction software version 10.7.3.1. Median signal intensities were cyclic lowess normalized. Technical replicates for each miRNA level were averaged using the median. Data was flagged if the raw signal intensity was less than the mean plus three standard deviations of the negative controls or if the median of the technical replicates was flagged to be in the background.
|
| |
|
| Submission date |
Oct 25, 2010 |
| Last update date |
Oct 19, 2011 |
| Contact name |
Andrew Williams |
| Organization name |
Health Canada
|
| Street address |
50 Columbine
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A OK9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL8824 |
| Series (2) |
| GSE24909 |
Lack of hepatic response of microRNA in mice following chronic benzo(a)pyrene exposure (miRNA) |
| GSE24910 |
Lack of hepatic response of microRNA in mice following chronic benzo(a)pyrene exposure |
|
