|
| Status |
Public on Jan 21, 2011 |
| Title |
pLPC_wt_E7_38_0C_rep_1 |
| Sample type |
RNA |
| |
|
| Source name |
pLPC wt E7 at 38oC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Human mammary fibroblasts (HMF3A)
treatment: pLPC wt E7 (viral protein HPV16 E7) grown at 38oC
|
| Treatment protocol |
HMF3A cells were cultured at 34°C ±0.5°C and temperature shift experiments performed at 38°C ±0.5°C. Cells were incubated at 38°C ±0.5°C for 7 days before RNA was extracted. HMF3A cells were serum starved by culturing in DMEM supplemented with 0.25% heat inactivated fetal bovine serum, 2mM glutamine, 100 units per ml penicillin and 100 micrograms per ml streptomycin. Cultures were fed with fresh medium the day prior to RNA extraction and were harvested at no greater than 80% confluence.
|
| Growth protocol |
HMF3A cells and derivatives thereof were maintained in DMEM supplemented with 10% heat inactivated fetal bovine serum, 2mM glutamine, 100 units per ml penicillin and 100 micrograms per ml streptomcyin and cultured in atmosphere of 5% CO2 and 20% O2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Invitrogen + Enzo
|
| |
|
| Hybridization protocol |
According to Affymetrix
|
| Scan protocol |
According to Affymetrix
|
| Description |
pLPC wt E7 (viral protein HPV16 E7) grown at 38oC
|
| Data processing |
The data were analyzed with Bioconductor R packages affy and limma using RMA as normalisation method.
|
| |
|
| Submission date |
Oct 19, 2010 |
| Last update date |
Jan 21, 2011 |
| Contact name |
Parmjit Jat |
| E-mail(s) |
[email protected]
|
| Phone |
(44) 20 78373973
|
| URL |
http://www.prion.ucl.ac.uk
|
| Organization name |
UCL
|
| Department |
Institute of Neurology
|
| Lab |
Neurodegenerative Disease
|
| Street address |
Queen Square
|
| City |
London |
| ZIP/Postal code |
WC1N 3BG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE24810 |
Dissecting the signalling pathways underlying cellular senescence |
|
