E12.5 DRGs from control and Erk1/2CKO(Wnt1) embryos derived from three independent litters were dissected in PBS supplemented with 10% RNAlater (Qiagen) and frozen on dry ice.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted with Trizol (Invitrogen) and a Qiagen RNeasy Mini kit per manufacturer’s instructions.