|
| Status |
Public on Dec 29, 2011 |
| Title |
3985-2 E2F1 Null |
| Sample type |
RNA |
| |
|
| Source name |
E2F1 Null
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
genotype: E2F1 null
tissue: mammary gland tumor
|
| Treatment protocol |
Tumors were flash frozen.
|
| Growth protocol |
All tumors are from MMTV-Myc transgenics in an FVB background.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from mouse mammary tumors using the Qiagen RNeasy Midi Kit.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
The resulting biotin-labeled RNA samples were then hybridized with Affymetrix Mouse 430A 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
3985-2
|
| Data processing |
The data were processed using Affymetrix Expression Console to generate MAS5 data.
|
| |
|
| Submission date |
Oct 08, 2010 |
| Last update date |
Dec 29, 2011 |
| Contact name |
Eran Andrechek |
| Phone |
517-884-5042
|
| Organization name |
Michigan State University
|
| Department |
Physiology
|
| Lab |
Andrechek
|
| Street address |
2120 BPS Building
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE24594 |
MMTV-Myc tumor development in E2F-null backgrounds |
|
