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Sample GSM606154 Query DataSets for GSM606154
Status Public on Dec 29, 2011
Title 3883-1 E2F WT
Sample type RNA
 
Source name E2F WT
Organism Mus musculus
Characteristics strain: FVB
genotype: wild-type
tissue: mammary gland tumor
Treatment protocol Tumors were flash frozen.
Growth protocol All tumors are from MMTV-Myc transgenics in an FVB background.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from mouse mammary tumors using the Qiagen RNeasy Midi Kit.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The resulting biotin-labeled RNA samples were then hybridized with Affymetrix Mouse 430A 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description 3883-1
Data processing The data were processed using Affymetrix Expression Console to generate MAS5 data.
 
Submission date Oct 08, 2010
Last update date Dec 29, 2011
Contact name Eran Andrechek
Phone 517-884-5042
Organization name Michigan State University
Department Physiology
Lab Andrechek
Street address 2120 BPS Building
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
 
Platform ID GPL8321
Series (1)
GSE24594 MMTV-Myc tumor development in E2F-null backgrounds

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 353.8192
AFFX-BioB-M_at 504.871
AFFX-BioB-3_at 364.7865
AFFX-BioC-5_at 1055.667
AFFX-BioC-3_at 1683.15
AFFX-BioDn-5_at 2835.236
AFFX-BioDn-3_at 4447.922
AFFX-CreX-5_at 14093.68
AFFX-CreX-3_at 13826.89
AFFX-DapX-5_at 1138.469
AFFX-DapX-M_at 1689.012
AFFX-DapX-3_at 1925.07
AFFX-LysX-5_at 113.8071
AFFX-LysX-M_at 139.0367
AFFX-LysX-3_at 328.1243
AFFX-PheX-5_at 148.0379
AFFX-PheX-M_at 186.4326
AFFX-PheX-3_at 195.5403
AFFX-ThrX-5_at 251.7155
AFFX-ThrX-M_at 320.8465

Total number of rows: 22690

Table truncated, full table size 455 Kbytes.




Supplementary file Size Download File type/resource
GSM606154.CEL.gz 2.0 Mb (ftp)(http) CEL
GSM606154.CHP.gz 201.0 Kb (ftp)(http) CHP
Processed data included within Sample table

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