|
| Status |
Public on Apr 19, 2022 |
| Title |
human-HUVEC-MAF-dox-2_S22 |
| Sample type |
SRA |
| |
|
| Source name |
HUVEC KO 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HUVEC
genotype: MAF KO
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
ECs were isolated by fluorescence-activated cell sorting and subjected to RNA extraction using RNeasy Mini Kit from Qiagen.
RNA-seq libraries of polyadenylated RNA were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) or TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
HUVEC MAF Knockout
|
| Data processing |
FastQC (v0.11.5) to check FASTQ quality.
Reads trimmed with Trimmomatic (v0.36)
TopHat2 v2.1.0; Bowtie2 v2.2.6 was used for alignment and genes quantified with HTSeq (v0.11.1)
Assembly: GRCh38
Supplementary files format and content: Gene count matrix
|
| |
|
| Submission date |
Apr 19, 2022 |
| Last update date |
Apr 21, 2022 |
| Contact name |
Jesus Maria Gomez Salinero |
| E-mail(s) |
[email protected]
|
| Organization name |
Weill Cornell Medicine
|
| Street address |
1300 York Avenue
|
| City |
NEW YORK |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (2) |
| GSE172361 |
Temporal and functional determinants of intra-hepatic vascular homeostatic specialization [human liver + HUVEC] |
| GSE172362 |
Temporal and functional determinants of intra-hepatic vascular homeostatic specialization |
|
| Relations |
| BioSample |
SAMN27642711 |
| SRA |
SRX14920384 |
