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| Status |
Public on Apr 21, 2022 |
| Title |
76 Hippocampus, 22M |
| Sample type |
SRA |
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| Source name |
Hippocampus
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| Organism |
Mus musculus |
| Characteristics |
strain: C57 BL/6
tissue: Hippocampus
Sex: Male
genotype: WT
treatment: aged
age: 22M
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| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted from young (3M) and aging (22M) hippocampus samples in mice by using Trizol reagents (Invitrogen). The extracted total RNA samples were subjected to agarose electrophoresis and Nanodrop for quality inspection and quantification.1-2g total RNA was selected from each sample for sequencing library construction.
Oligo magnetic beads were used to enrich the mRNA, and the KAPA Stranded RNA-Seq Library Prep Kit (Illumina) was used to construct the library
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
For read alignment, the software Hisat2 was used.
For read quantification/FPKM calculation, the software Ballgown was used.
Assembly: GRCm38 (mm10)
Supplementary files format and content: Excel file with FPKM values for each Sample
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| Submission date |
Apr 19, 2022 |
| Last update date |
Apr 21, 2022 |
| Contact name |
Qi Shang |
| E-mail(s) |
[email protected]
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| Phone |
15975473862
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| Organization name |
Guangzhou University of Chinese Medicine
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| Street address |
No.16, Airport road, BaiYun Road, Guangzhou City,
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| City |
Guangzhou |
| State/province |
Guangzhou |
| ZIP/Postal code |
510405 |
| Country |
China |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE201029 |
Myristic acid alleviates hippocampal aging via regulating GABAergic expressions |
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| Relations |
| BioSample |
SAMN27629969 |
| SRA |
SRX14908247 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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