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Status
Public on Jun 06, 2006
Title
WT (yw) irradiated replicate 1
Sample type
RNA
Source name
embryos, WT (yw), irradiated
Organism
Drosophila melanogaster Characteristics
Wild type (yw), embryos 4-6.5 hours after egg laying, irradiated
Extracted molecule
total RNA
Extraction protocol
Approximately 1 gram of wild-type (yw strain) embryos was collected 0-2.5 hours after egg laying. The embryos were aged for 2.5 hours at 25C, treated with 40Gy of gamma radiation, and allowed a 1.5 hour recovery prior to dechorionation. Total RNA was isolated using Trizol (Invitrogen).
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
irradiated Wild type (yw) embryos
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Jun 08, 2005
Last update date
Jun 08, 2005
Contact name
John M Abrams
E-mail(s)
[email protected] Phone
214-648-9226
Fax
214-648-8694
Organization name
University of Texas Southwestern Medical Center
Department
Cell Biology
Lab
Abrams
Street address
5323 Harry Hines Blvd
City
Dallas
State/province
TX
ZIP/Postal code
75390-9039
Country
USA
Platform ID
GPL72 Series (1)
GSE2780 Radiation-induced Dmp53-dependent expression profile
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE 141200_at
78.4
A
0.318883
141201_at
31.4
A
0.921094
141202_at
500.9
P
0.001755
141203_at
149.3
P
0.023995
141204_at
291
P
0.004591
141205_at
121.6
P
0.036797
141206_at
867.1
P
0.006575
141207_at
167.1
P
0.002143
141208_at
342
P
0.001432
141209_at
1727.7
P
0.000944
141210_at
105.9
A
0.216313
141211_at
355.2
P
0.000944
141212_at
2333.7
P
0.000491
141213_at
20.4
A
0.413046
141214_at
735.7
P
0.000613
141215_at
468.9
P
0.03202
141216_at
545.8
P
0.012921
141217_at
605.1
P
0.005504
141218_at
1285.3
P
0.000491
141219_at
127.4
P
0.023995
14010 360 Kbytes .Supplementary file Size Download File type/resource GSM60481.CEL.gz
3.1 Mb
(ftp) (http) CEL
GSM60481.EXP.gz
474 b
(ftp) (http) EXP
