|
| Status |
Public on Apr 07, 2011 |
| Title |
DMTU,rep1 |
| Sample type |
RNA |
| |
|
| Source name |
T87 suspension cells
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
cell type: suspension cells
age: 5 days
time: 20 hours
hormone: ethanol (control)
inhibitor: Dimethylthiourea (DMTU)
genetic background: Col-0
cell line: T87
|
| Treatment protocol |
ABA and DMTU were added to the medium containing the suspension cells to the final required concentrations of 50 µM and 5mM, respectively. Treatments were continued for 20 h before RNA extraction
|
| Growth protocol |
Arabidopsis thaliana (Columbia ecotype) T87 suspension cells were obtained from RIKEN BioResource Center (Tsukuba, Japan) and were cultured at 20 °C under 16 h light, 8h dark illumination (24 μE s−1 m−2) with shaking at 120 rpm. Cells were subcultured every 7 days by adding 10 ml of the cell suspension to a 500 ml flask containing 90 ml of Gamborg B5 medium with 3% sucrose and 0.5 µM 1-naphthaleneacetic acid (NAA). Experiments were carried out in the exponential phase of growth at about 120 h of cell cultivation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the RNeasy Plant Mini Kit (Qiagen) following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix 3' IVT labeling kit
|
| |
|
| Hybridization protocol |
Arabidopsis ATH1 oligonucleotide-based genome arrays were used, and hybridization and washing were performed by using the standard Affymetrix protocol for RNA analyses (www.affymetrix.com/products/arrays/specific/arab.affx).
|
| Scan protocol |
Affymetrix 7G scanner
|
| Data processing |
RMA (Limma)
|
| |
|
| Submission date |
Oct 05, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Maik Boehmer |
| E-mail(s) |
[email protected]
|
| Phone |
+1-858-534-7432
|
| Fax |
+1-858-534-7108
|
| Organization name |
University of Caifornia, San Diego
|
| Department |
Biology
|
| Lab |
Julian I. Schroeder
|
| Street address |
9500 Gilman Dr 0116
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE23301 |
Quantitative transcriptomic analysis of abscisic acid-induced and reactive oxygen species-dependent expression changes and proteomic profiling in Arabidopsis suspension cells |
|
| Relations |
| Reanalyzed by |
GSE118579 |
