|
| Status |
Public on Sep 29, 2010 |
| Title |
MM#34_17-08 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
purified CD138+ plasma cells from myeloma patient
|
| Organism |
Homo sapiens |
| Characteristics |
age: 60 yrs
Sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MicroRNA was isolated from samples using Trizol (Invitrogen, Paisley, UK) and RNeasy columns as described by the manufacturer (Qiagen, Crawley, UK).
|
| Label |
Cy3
|
| Label protocol |
Enriched microRNA (500ng) samples were labeled with either Cy3 or Cy5 dyes using an Array 900microRNA direct kit from Genisphere (Hatfield, PA)
|
| |
|
| Channel 2 |
| Source name |
tonsil
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: pooled tonsils (n=12)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MicroRNA was isolated from samples using Trizol (Invitrogen, Paisley, UK) and RNeasy columns as described by the manufacturer (Qiagen, Crawley, UK).
|
| Label |
Cy5
|
| Label protocol |
Enriched microRNA (500ng) samples were labeled with either Cy3 or Cy5 dyes using an Array 900microRNA direct kit from Genisphere (Hatfield, PA)
|
| |
|
| |
| Hybridization protocol |
Hybridization protocol Samples were hybridised using the Array 900microRNA direct kit from Genisphere (Hatfield, PA) as described by the manufacturer on a A-hyb machine (Miltenyi Biotec)
|
| Scan protocol |
Slides were scanned on a Perkin-Elmer ArrayExpress scanner
|
| Data processing |
Image analysis was carried out with BlueFuse software (BlueGnome, Cambridge, UK). Raw image data were global LOESS-normalized within arrays and normalized between arrays using the LIMMA package. The normalized log ratios (average of four replicates per probe) were used for subsequent analysis in Genespring 7.2 (Agilent Technologies, CA, US).
|
| |
|
| Submission date |
Sep 25, 2010 |
| Last update date |
Sep 28, 2010 |
| Contact name |
charles lawrie |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Oxford
|
| Department |
NDCLS
|
| Street address |
John Radcliffe Hospital
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10385 |
| Series (1) |
| GSE24371 |
MicroRNA Expression in Multiple Myeloma is Associated with Genetic Subtype, Isotype and Prognostic Outcome |
|
