|
| Status |
Public on Sep 25, 2010 |
| Title |
nas4x-1 roots + Fe, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
nas4x-1, Roots grown under + Fe
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: Roots
|
| Treatment protocol |
No special treatment has been applied
|
| Growth protocol |
Wild-type and nas4x-1 plants were grown in hydroponic solution containing a quarter strength of Hoagland salts supplied with 10µM FeNa-EDTA. The medium was exchanged weekly. Four weeks after germination, plants were divided into two groups, one of them was kept in the same conditions as mentioned above (10µM FeNa-EDTA, + Fe) while the other group was transferred to Hoagland solution without Fe (0 µM FeNa-EDTA, - Fe). Cultivation took place at 21°C/19°C and 16-h-light, 8-h-dark cycles and a light intensity of 150 µmol x m-2 x s-1.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 100 mg of root and leaf material with the Quiagen RNeasy Plant Mini Prep Kit.
|
| Label |
Biotin
|
| Label protocol |
5 µg RNA of each sample were processed into biotin-labeled cRNA and hybridized to Affymetrix GeneChip Arabidopsis ATH1 Genome Arrays (Affymetrix, High Wycombe, U.K.), using the Affymetrix One-Cycle Labeling and Control (Target) kit
|
| |
|
| Hybridization protocol |
(see label protocol)
|
| Scan protocol |
Affymetrix GeneChip scanner 3000
|
| Description |
Roots of quadruple nasT-DNA insertionmutant in background of ecotype Columbia, grown under Fe supply
|
| Data processing |
Data were processed by Affymetrix GeneChip Operating software (GCOS) and quantile normalized using R.
|
| |
|
| Submission date |
Sep 24, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Mara Schuler |
| Organization name |
Saarland University
|
| Street address |
Campus SB, Geb.A2 4
|
| City |
Saarbrücken |
| ZIP/Postal code |
66123 |
| Country |
Germany |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE24348 |
Expression data of Arabidopsis thaliana wild-type plants and quadruple nas T-DNA insertion mutants grown under different Fe supply conditions |
|
| Relations |
| Reanalyzed by |
GSE118579 |
