|
| Status |
Public on Oct 31, 2010 |
| Title |
Male PBS_rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
age: 11 weeks, Adrenalectomized
tissue: liver
treatment group: vehicle (phosphate buffered saline) for 6 hours
|
| Treatment protocol |
Male and female (diestrous 1) adrenalectomized C57BL/6 mice were treated with vehicle (phosphate buffered saline) or dexamethasone (1 mg/kg i.p.) (Steraloids) on the same day. Six hours after dexamethasone treatment the liver was harvested and kept in RNAlater solution at 4oC overnight. All samples were frozen and stored at -800C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Livers were homogenized and total RNA from liver was extracted by using the RNeasy Midi Kit (Qiagen) according to the manufacturer’s instructions. All samples were treated with the RNase-free DNase set (Qiagen). Samples were aliquoted and frozen at -800C.
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
Male Control - replicate 2
Agilent TIFF High File: US23502387_251486824229_S01_H.tif
Agilent TIFF Low File: US23502387_251486824229_S01_L.tif
|
| Data processing |
Data were obtained and processed with the Agilent Feature Extraction Software (v9.5) and the resulting data were imported into Rosetta Resolver (v7.1).
|
| |
|
| Submission date |
Sep 21, 2010 |
| Last update date |
Sep 21, 2010 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE24256 |
Liver gene expression analysis of male and female C57BL/6 mice treated with glucocorticoid. |
|
