|
| Status |
Public on Mar 14, 2022 |
| Title |
M w 0S 3 |
| Sample type |
SRA |
| |
|
| Source name |
w1118 Male
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: w1118
Sex: male
tissue: Whole larva
developmental stage: 108 AEL
diet: 0S
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10 108hr (AEL) male and female larvae were removed from Drosophila diet (1S or 0S) and flash frozen on dry ice. RNA was isolated using Trizol reagent. Quality control of samples was performed using the Agilent 2100 Bioanalyzer to confirm sample quality.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Sequencing was performed using on a NextSeq 500 sequencer.
Sequence quality was assessed using FastQC on Illumina BaseSpace
Reads were aligned to Drosophila melanogaster genome (UCSC dm3) using STAR aligner with default settings
For differential expression analysis, fragments per kilobase of transcript per million mapped reads were calculated using the DESeq2 normalization method
Log fold changes and significance for differential expression were determined and a significance threshold of 0.05 was selected for adjusted p values determined with Bonferroni correction
Assembly: UCSC dm3
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
|
| |
|
| Submission date |
Mar 11, 2022 |
| Last update date |
Mar 18, 2022 |
| Contact name |
Elizabeth Rideout |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of British Columbia
|
| Department |
Cellular and Physiological Sciences
|
| Street address |
2350 Health Sciences Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE198435 |
A low sugar diet enhances Drosophila body size in males and females via sex-specific mechanisms |
|
| Relations |
| BioSample |
SAMN26582629 |
| SRA |
SRX14439235 |
