|
| Status |
Public on Sep 03, 2010 |
| Title |
Thymic tumor from mouse RJ27 |
| Sample type |
RNA |
| |
|
| Source name |
transformed T cells from a mouse thymic lymphoma
|
| Organism |
Mus musculus |
| Characteristics |
age: 8-10 weeks
disease state: lymphoma
tissue: thymus
cell type: transformed T cells
genotype/variation: IkL/L Notch1f/f CD4-Cre+
|
| Treatment protocol |
Thymic lymphomas were dissected from euthanized sick mice and tumor cells collected after tissue disruption between frosted ends of microscope slides
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were extracted with the RNAeasy kit, following the manufacturer's instructions (including DNAse I treatment)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of cRNA were hybridized for 16 hr at 45C on GeneChip 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned with a GeneChip® Scanner 3000 7G System
|
| Description |
gene expression data from a thymic tumor from an Ikaros deficient thymic lymphoma with a Notch promoter/exon1 deletion
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. Target intensity was set at 100.
|
| |
|
| Submission date |
Sep 03, 2010 |
| Last update date |
Dec 05, 2013 |
| Contact name |
Susan Chan |
| E-mail(s) |
[email protected]
|
| Phone |
33 3 88 65 34 72
|
| Organization name |
IGBMC
|
| Street address |
1, rue Laurent Fries
|
| City |
Illkirch |
| ZIP/Postal code |
67404 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE23972 |
Ikaros deficient mouse thymic lymphomas |
|
