|
| Status |
Public on Sep 17, 2011 |
| Title |
NaBu 2, 16hrs |
| Sample type |
RNA |
| |
|
| Source name |
E14 embryonic stem cells, NaBu
|
| Organism |
Mus musculus |
| Characteristics |
cell line: E14
cell type: embryonic stem cells (ESCs)
treatment: sodium butyrate (NaBu)
treatment time: 16 hours
|
| Treatment protocol |
0.5mM VPA or 0.2mM NaBu were added to the cell medium in the indicated samples for 16 hrs.
|
| Growth protocol |
Cells were grown on mitomycin-C-treated primary embryonic fibroblasts, with ES cell medium with 15% FBS and leukemia inhibitory factor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
According to manufacturer's instructions at the Genomics Center at the Life Sciences Institute, The Hebrew University of Jerusalem.
|
| |
|
| Hybridization protocol |
According to manufacturer's instructions at the Genomics Center at the Life Sciences Institute, The Hebrew University of Jerusalem.
|
| Scan protocol |
GeneChip Scanner 3000 7G according to manufacturer's instructions at the Genomics Center at the Life Sciences Institute, The Hebrew University of Jerusalem.
|
| Description |
Replicate 2 of 2.
|
| Data processing |
RMA signal intensity calculation using Affymetrix Expression Console, version 1.1.
|
| |
|
| Submission date |
Sep 02, 2010 |
| Last update date |
Sep 17, 2011 |
| Contact name |
Eran Meshorer |
| E-mail(s) |
[email protected]
|
| Phone |
+972-2-6585161
|
| Fax |
+972-2-6586073
|
| URL |
http://meshorerlab.huji.ac.il/
|
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Genetics
|
| Lab |
Stem cell chromatin
|
| Street address |
Edmond J. Safra campus
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE23956 |
Gene expression profiles of E14 ESCs treated with low levels of HDAC inhibitors for 16 hrs |
| GSE23958 |
Gene expression and H3K9ac genome-wide maps following HDAC inhibition in mouse ES cells |
|
