|
| Status |
Public on Jul 01, 2013 |
| Title |
DCD3B |
| Sample type |
RNA |
| |
|
| Source name |
Liver Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
trasplant donor origin: Donor after cardiac death (DCD)
time: B
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The tissue was cut after extraction into 2 mm2 pieces and immediately stored in diethyl pyrocarbonate (DEPC) solution, a nuclease inhibitor. In less than 24 hours the tissue was frozen and stored at -80º C. RNA extraction: Total RNAs were prepared from the lysates of liver with Trizol reagent (Invitrogen) according to the instructions provided by the manufacturer. RNA was treated with TURBO DNA-free Kit to remove DNA contamination according to the instructions provided by the manufacturer (Ambion, Austin, TX).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared with the Illumina® TotalPrep™ RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Liver sample DCD3B
|
| Data processing |
The data were normalised using robust spline normalisation with Bioconductor (v:2.5) Lumi package
|
| |
|
| Submission date |
Aug 16, 2010 |
| Last update date |
Jul 01, 2013 |
| Contact name |
Juanjo Lozano |
| E-mail(s) |
[email protected]
|
| Organization name |
CIBEREHD
|
| Department |
Plataforma de Bioinformatica
|
| Street address |
C/ Rosselló 153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE23649 |
GENE-EXPRESSION PROFILES OF HUMAN LIVER BIOPSIES CORRELATE WITH POST-TRANSPLANT ALLOGRAFT FUNCTION AND ARE DETERMINED BY DONOR TYPE. |
|
