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Sample GSM577725 Query DataSets for GSM577725
Status Public on Aug 01, 2011
Title Arabidopsis VND7XVE Control 2
Sample type RNA
 
Source name Arabidopsis protoplasts
Organism Arabidopsis thaliana
Characteristics genotype/variation: vnd7xve
treatment group: cycloheximide
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the QIAGEN Plant RNA isolation kit.
Label Biotin
Label protocol Affymetrix One-Cycle Eukaryotic Target Labeling kit.
 
Hybridization protocol Standard Affymetrix GeneChip Technology.
Scan protocol GeneChips were scanned using the GeneArray Scanner 3000.
Data processing Expression values are calculated based on the difference of the PM signal and MM signal at each of the probe pairs using the Affymetrix GeneChip Operating System (GCOS). This software employs statistical algorithms to calculate qualitative and quantitative expression values for each transcript. It also generates a statistical p-value, which suggests the level of confidence in each of the qualitative measurements.
 
Submission date Aug 10, 2010
Last update date Aug 01, 2011
Contact name Zheng-Hua Ye
Organization name University of Georgia
Department Plant Biology
Street address 2502 Miller Plant Sciences
City Athens
State/province GA
ZIP/Postal code 30602
Country USA
 
Platform ID GPL198
Series (1)
GSE23555 Identification of Direct Targets of SND1 and VND7

Data table header descriptions
ID_REF
VALUE MAS5 Detection signal
ABS_CALL =Indication of whether the transcript is present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 245.3 P 0.0001
AFFX-BioB-M_at 352.6 P 0.0000
AFFX-BioB-3_at 252.9 P 0.0001
AFFX-BioC-5_at 806.0 P 0.0000
AFFX-BioC-3_at 937.2 P 0.0000
AFFX-BioDn-5_at 1600.4 P 0.0000
AFFX-BioDn-3_at 2254.8 P 0.0000
AFFX-CreX-5_at 10477.5 P 0.0000
AFFX-CreX-3_at 15784.6 P 0.0000
AFFX-DapX-5_at 792.3 P 0.0001
AFFX-DapX-M_at 1211.3 P 0.0001
AFFX-DapX-3_at 2144.2 P 0.0000
AFFX-LysX-5_at 69.4 P 0.0000
AFFX-LysX-M_at 192.7 P 0.0004
AFFX-LysX-3_at 307.0 P 0.0000
AFFX-PheX-5_at 143.5 P 0.0001
AFFX-PheX-M_at 234.8 P 0.0003
AFFX-PheX-3_at 313.9 P 0.0001
AFFX-ThrX-5_at 169.2 P 0.0003
AFFX-ThrX-M_at 327.6 P 0.0001

Total number of rows: 22810

Table truncated, full table size 547 Kbytes.




Supplementary file Size Download File type/resource
GSM577725.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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