|
| Status |
Public on Feb 25, 2011 |
| Title |
Genital_disc_L3_male_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophlia genital discs of male late third-instar larvae (L3)
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: dissected genital disc
genotype: esgGAL4.B, UAS-GFP.nls/CyO
developmental stage: late third-instar larva (L3)
sex: male
|
| Treatment protocol |
Larvae or pupae were dissected in cold PBS and immediately transferred to the RNA extraction protocol's lysis buffer.
|
| Growth protocol |
Flies were reared at 25C on standard cornmeal-molasses-agar medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Ambion MELT kit then further purified and concentrated using Qiagen RNeasy MinElute columns, following each manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was produced from 20 ng of total RNA using the NuGEN Ovation Biotin System, following the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
For each sample, 1.5 µg biotinylated cDNA was hybridized to an Affymetrix GeneChip Drosophila Genome 2.0 Array and washed and stained using the Affymetrix Fluidics Station 450 following manufacturer's protocols.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from Drosophlia genital discs of male late third-instar larvae (L3)
|
| Data processing |
The data were background-corrected and normalized using RMA as implemented in the R/Bioconductor package.
|
| |
|
| Submission date |
Jul 30, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Mark L Siegal |
| E-mail(s) |
[email protected]
|
| Organization name |
New York University
|
| Department |
Department of Biology, Center for Genomics and Systems Biology
|
| Street address |
100 Washington Sq E
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10003 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE23344 |
Expression data from Drosophila female or male genital discs at different time points during morphogenesis |
|
| Relations |
| Reanalyzed by |
GSE119084 |
