|
| Status |
Public on Jun 21, 2022 |
| Title |
BM cells, Mx1-Cre Sf3b1-K700E_rep3 |
| Sample type |
SRA |
| |
|
| Source name |
Lineage- cKit+ bone marrow cells in femora and tibiae
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
donor genotype: CD45.2(+) Mx1-Cre Sf3b1-K700E
recipient genotype: CD45.1(+) CD45.2(-)
timing of preparation: 6 months after pIpC injection
tissue: bone marrow
|
| Treatment protocol |
Four weeks after BMT, the recipient mice received 20 mg/kg pIpC injection every other day for a total of 3 doses.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy columns (Qiagen) per manufacturer’s instructions.
RNA was poly(A)-selected, and unstranded Illumina libraries were prepared with the standard TruSeq protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
bcl2fastq
nf-core/rnaseq 3.0
Genome_build: mm10
Supplementary_files_format_and_content: gene expression (counts, TPMs, FPKMs)
|
| |
|
| Submission date |
Dec 10, 2021 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Masaki Nomura |
| E-mail(s) |
[email protected]
|
| Organization name |
Foundation for Biomedical Research and Innovation at Kobe
|
| Street address |
6-3-7 Minatojima Minamimachi, Chuo Ward
|
| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE190655 |
Aberrant EVI1 splicing contributes to EVI1-rearranged leukemia [mouse_RNA-seq] |
| GSE190656 |
Aberrant EVI1 splicing contributes to EVI1-rearranged leukemia |
|
| Relations |
| BioSample |
SAMN23887337 |
| SRA |
SRX13377210 |
