|
| Status |
Public on Jun 21, 2022 |
| Title |
Kp-HW |
| Sample type |
SRA |
| |
|
| Source name |
hematopoietic cells (K562 cells)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: chronic myelogeneous leukemia cell
treatment (transfection): pMYs-IG_3xHA-EVI1-WT
expression type: stably expressed
expression level: retroviral overexpression
cell line: K562
|
| Growth protocol |
K562 cells were cultured with RPMI-1640 supplemented with 10% fetal bovine serum, 100 u/ml penicillin, and 100 µg/ml streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy mini kit (Qiagen) following the manufacturer’s protocol
TruSeq RNA Library Kit v2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
bcl2fastq
nf-core/rnaseq 3.0
Genome_build: hg19
Supplementary_files_format_and_content: gene expression (counts, TPMs, FPKMs)
|
| |
|
| Submission date |
Dec 10, 2021 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Masaki Nomura |
| E-mail(s) |
[email protected]
|
| Organization name |
Foundation for Biomedical Research and Innovation at Kobe
|
| Street address |
6-3-7 Minatojima Minamimachi, Chuo Ward
|
| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE190653 |
Aberrant EVI1 splicing contributes to EVI1-rearranged leukemia [K562_RNA-seq] |
| GSE190656 |
Aberrant EVI1 splicing contributes to EVI1-rearranged leukemia |
|
| Relations |
| BioSample |
SAMN23887323 |
| SRA |
SRX13377254 |
