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Sample GSM569411 Query DataSets for GSM569411
Status Public on Jan 18, 2011
Title preimmune_seq
Sample type SRA
 
Source name hES h1 cells, preimmune
Organism Homo sapiens
Characteristics cell type: H1 human embryonic stem cells
chromosome: diploid
passages: 30-50
sample type: pre_IP
antibody: n/a
Growth protocol H1 hESCs were cultured in Matrigel-coated plates in mouse embryonic fibroblast conditioned medium supplemented with 8 ng/mL bFGF
Extracted molecule total RNA
Extraction protocol Lin28 immunoprecipitated or preimmune RNAs were individually extracted and libraries were prepared using the Illumina mRNA-Seq Sample Prep Kits (P/N 1004814) according to the manufacturer’s instructions
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer II
 
Description preimmune RNA
RNAseq
total reads: 9496883
uniquely mapped reads with up to 2 mismatches: 4746125
Data processing Alignment: Sequence reads were obtained and mapped to the human hg18 (March, 2006) genomes by using bowtie with uniquely mapping and up to 2 mismatches
Column description in the bedgraph files: Chromosome; start position; end position; height
 
Submission date Jul 23, 2010
Last update date May 15, 2019
Contact name Ling-Ling Chen
E-mail(s) [email protected]
Organization name Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Street address 320 Yue-Yang Road
City Shanghai
ZIP/Postal code 200031
Country China
 
Platform ID GPL9115
Series (1)
GSE23109 Genome-wide analysis of RNAs associated with Lin28
Relations
SRA SRX024346
BioSample SAMN00017772

Supplementary file Size Download File type/resource
GSM569411_preimmune.bedgraph.gz 18.1 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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