|
Status |
Public on Jan 18, 2011 |
Title |
preimmune_seq |
Sample type |
SRA |
|
|
Source name |
hES h1 cells, preimmune
|
Organism |
Homo sapiens |
Characteristics |
cell type: H1 human embryonic stem cells chromosome: diploid passages: 30-50 sample type: pre_IP antibody: n/a
|
Growth protocol |
H1 hESCs were cultured in Matrigel-coated plates in mouse embryonic fibroblast conditioned medium supplemented with 8 ng/mL bFGF
|
Extracted molecule |
total RNA |
Extraction protocol |
Lin28 immunoprecipitated or preimmune RNAs were individually extracted and libraries were prepared using the Illumina mRNA-Seq Sample Prep Kits (P/N 1004814) according to the manufacturer’s instructions
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer II |
|
|
Description |
preimmune RNA RNAseq total reads: 9496883 uniquely mapped reads with up to 2 mismatches: 4746125
|
Data processing |
Alignment: Sequence reads were obtained and mapped to the human hg18 (March, 2006) genomes by using bowtie with uniquely mapping and up to 2 mismatches Column description in the bedgraph files: Chromosome; start position; end position; height
|
|
|
Submission date |
Jul 23, 2010 |
Last update date |
May 15, 2019 |
Contact name |
Ling-Ling Chen |
E-mail(s) |
[email protected]
|
Organization name |
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
|
Street address |
320 Yue-Yang Road
|
City |
Shanghai |
ZIP/Postal code |
200031 |
Country |
China |
|
|
Platform ID |
GPL9115 |
Series (1) |
GSE23109 |
Genome-wide analysis of RNAs associated with Lin28 |
|
Relations |
SRA |
SRX024346 |
BioSample |
SAMN00017772 |