|
| Status |
Public on Jan 05, 2011 |
| Title |
Ppif-/- heart 2 |
| Sample type |
RNA |
| |
|
| Source name |
Heart sample from 8-week-old Ppif-/- mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Ppif-/- (global gene knockout)
tissue: heart
age: 8 weeks
|
| Treatment protocol |
Mice were euthanized and hearts were rapidly removed and placed in RNAeasy for 48hr at 4C in preparation for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Fibrous Tissue kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The Affymetrix Whole Transcript Sense Target Labeling Assay (Affymetrix) is used to create biotin-labeled sense-strand cDNA targets for hybridization to the Gene 1.0 ST Arrays.
|
| |
|
| Hybridization protocol |
Hybridization cocktail for a single probe array contained 2.5 ug of fragmented cRNA, 50 pM Control Oligonucleotide B2 (Affymetrix), 20X Eukaryotic Hybridization Controls (1.5 pM bioB, 5 pM bioC, 25 pM bioD, 100 pM cre) (Affymetrix), 7% DMSO, and 1X Hybridization Buffer. GeneChip Mouse Gene 1.0 ST Array (Affymetrix) was hybridized at 45C for 18 hours in the GeneChip Hybridization Oven 640 (Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from Ppif-/- mouse hearts.
Ppif-/- mice were back-crossed to C57BL/6 from an original Sv129 background. See original description in Baines et al. Nature. 2005; 434:658-662 (PMID 15800627).
|
| Data processing |
The data was RMA normalized using GeneSpring GX 7.3.
|
| |
|
| Submission date |
Jul 20, 2010 |
| Last update date |
Jan 05, 2011 |
| Contact name |
John W Elrod |
| Organization name |
Cincinnati Children's Hospital and Medical Center
|
| Department |
Molecular Cardiovascular Biology
|
| Lab |
Molkentin
|
| Street address |
240 Albert Sabin Way
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
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